Conjunctival and eyelid swabs were collected from the right eye of the rabbits at the beginning of the study, before the intervention. Sterile cotton swabs were moistened with sterile normal saline which werethen wiped along the lower fornix of the right eye by everting the eyelid. During sampling, any contact of the swabs with eyelashes, eyelid skin or lacrimal punctum was avoided. A separate sterile swab was used for each eyelid specimen. Then the swabs were immediately streaked onto a blood agar plate. The plates were incubated at 37ºC for 24 hours. After 24 hours, the cultures were examined. If there was not enough growth of the colonies, they were reincubated for a further 24 hours. After 48 hours, the isolated colonies were identified by cultural characteristics and gram staining. By performing this phase, before instillation of the onion juice, we were able to find out more about the most common organisms that frequently inhabit the conjunctiva and eyelid of healthy rabbits. These results were then used as control data to compare with the results obtained after onion juice administration.
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